Dynamic light scattering machine software#
Moreover, DYNAMICS ® software contains an extensive, built-in solvent library with the most common solvents and buffer systems, including the option to create custom solvents. Solvent viscosity and refractive index are generally well-known and tabulated.The only necessary input parameters are solution viscosity and refractive index.ĭLS is a technique that can be readily applied for sizing unknown samples: The resulting intensity fluctuations in the light scattering detector are analyzed to determine a translational diffusion coefficient, which is converted to hydrodynamic radius via the Stokes-Einstein relationship (see sidebar). The sample is illuminated with a laser beam, and light waves scattered from the particles interfere constructively and destructively as the particles diffuse randomly. Pre-screening: Ideal to check sample quality before running other expensive and time-consuming biophysical methods such as TEM, AUC, SAXS/SANS, FFF and SEC.įor in-depth characterization of high-resolution size distributions and other physical properties, SEC-MALS and FFF-MALS offer complementary and orthogonal techniques to DLS.ĭLS determines the hydrodynamic size of a nanoparticle in solution by measuring the time scale of Brownian motion.Advanced properties: Measure particle concentration, nonspecific interactions ( A 2 or B 22) and differentiate between conformational changes and aggregation with the static light scattering option in the NanoStar and Plate Reader.Versatility: Beyond size and size distributions, measure other properties such as stability, stress-induced aggregation and molecular interactions.No shearing or filtering: always measure what’s in your solution.Low sample volume: Only 2 µL in the DynaPro NanoStar using quartz cuvettes, 4 µL in disposable cuvettes or standard microwell plates in the DynaPro Plate Reader.Non-destructive and fully recoverable: Samples may be transferred to other assays.
Dynamic light scattering machine full#
Full nanometer size range: from 0.5 nm to 2.5 µm in a single measurement, with a resolution up to a factor of 3-5 in size.
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